Sickle-Cell Anemia: Molecular and Cellular Bases of Therapeutic Approaches

Prostate cancer is the most frequently diagnosed cancer in men in Western countries. Clinically localized disease can be cured with surgery or radiotherapy, but once the disease has advanced or spread, there are no curative treatments. The aim of this thesis is to compare protein expression in a pair of normal epithelial and prostate cancer cells derived from the same patient and to determine the effect of interferon y on this expression pattern. Proteins that are differentially expressed or change in expression in response to y interferon might be of clinical value as biomarkers or therapeutic targets. Biotin labelling followed by avidin chromatography was used to obtain membrane protein enriched lysates from a pair of cell lines derived from normal epithelium (1542 NPX) and prostate cancer (1542 CP3TX) from the same patient. These proteins were resolved and identified using SDS PAGE, coomassie staining and mass spectrometry. The same protocol was used to identify proteins differentially expressed on stimulation with interferon y. The proteins identified were subject to further analysis, particularly annexin II (All), which was interferon-regulated. The expression of this protein was down regulated in the ... (continues)