What question did this study set out to answer?

To determine whether macrophage Fc receptor isoforms mediate polarized sorting and transcellular IgG transport when expressed in epithelial cells.

December 1, 1989The Journal of Cell BiologyOpen Access

Expression of macrophage-lymphocyte Fc receptors in Madin-Darby canine kidney cells: polarity and transcytosis differ for isoforms with or without coated pit localization domains.

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Authors

WHWalter HunzikerAgency for Science, Technology and Research IMIra MellmanUniversity of Geneva

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Implication

In vitro study reveals isoform-specific polarity and apical-to-basolateral IgG transcytosis of Fc receptors in epithelial cells, indicating a mechanism for placental antibody transport.

Key Points

To determine whether macrophage Fc receptor isoforms mediate polarized sorting and transcellular IgG transport when expressed in epithelial cells.
Transfected cDNA encoding mouse FcRII-B1 and FcRII-B2 isoforms into Madin-Darby canine kidney (MDCK) epithelial cells cultured on permeable filter supports.
Evaluated polarized surface distribution, lysosomal degradation, and bidirectional transcytosis using immune complexes and monovalent Fab fragments.
FcRII-B1 localized predominantly to the apical plasma membrane, whereas FcRII-B2 sorted predominantly to the basolateral plasma membrane.
FcRII-B2 mediated unidirectional transcellular transport of IgG complexes from the apical to basolateral domain, with weaker apical-to-basolateral transport by FcRII-B1 and no transport detected in the reverse direction.
FcRII-B2 transported monovalent Fab fragments across the cell layer without requiring receptor cross-linking, while also routing ligand internalized from either surface domain to lysosomes.

Cite This Study

Hunziker et al. (1989) studied this question.

synapsesocial.com/papers/6a700487febe604dd708d487 https://doi.org/10.1083/jcb.109.6.3291

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Implication

In vitro study reveals isoform-specific polarity and apical-to-basolateral IgG transcytosis of Fc receptors in epithelial cells, indicating a mechanism for placental antibody transport.

Expression of macrophage-lymphocyte Fc receptors in Madin-Darby canine kidney cells: polarity and transcytosis differ for isoforms with or without coated pit localization domains.

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Implication

Key Points

Cite This Study

Expression of macrophage-lymphocyte Fc receptors in Madin-Darby canine kidney cells: polarity and transcytosis differ for isoforms with or without coated pit localization domains.

Discussion

Member takes

Implication

Key Points

Cite This Study

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