What question did this study set out to answer?

To clone, determine the genomic organization of, and characterize the developmental expression profile of the novel murine homeobox gene Hesx1.

February 1, 1995Journal of Biological ChemistryOpen Access

Sequence, Genomic Organization, and Expression of the Novel Homeobox Gene Hesx1

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Authors

PTPaul Q. ThomasSouth Australian Health and Medical Research Institute BJBrett V. JohnsonThe University of Adelaide JRJoy RathjenUniversity of Tasmania

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Implication

Laboratory study reveals early embryonic expression of the novel homeobox gene Hesx1 in mice, suggesting a regulatory role in developmental patterning and fetal hematopoiesis.

Key Points

To clone, determine the genomic organization of, and characterize the developmental expression profile of the novel murine homeobox gene Hesx1.
Isolated and cloned cDNA sequences of Hesx1 from murine pluripotent embryonic stem cells.
Analyzed Hesx1 transcripts and tissue-specific expression patterns across undifferentiated embryonic stem cells, differentiating cells, embryonic tissues, and adult mouse tissues.
Hesx1 encodes an identical 185-amino-acid protein across two transcripts (1.0 and 1.2 kilobases) that diverge in their 3'-untranslated regions due to differential splice donor site utilization.
The Hesx1 homeodomain shares 80% sequence identity with Xenopus XANF-1 and less than 50% identity with other homeodomains, defining the founder members of a new homeodomain class.
Hesx1 expression is downregulated upon embryonic stem cell differentiation, is present in embryonic liver, viscera, amnion, and yolk sac, and is undetectable in adult mouse tissues.

Cite This Study

Thomas et al. (1995) studied this question.

synapsesocial.com/papers/6a70096835aa2c282ce155bc https://doi.org/10.1074/jbc.270.8.3869

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Implication

Laboratory study reveals early embryonic expression of the novel homeobox gene Hesx1 in mice, suggesting a regulatory role in developmental patterning and fetal hematopoiesis.

Sequence, Genomic Organization, and Expression of the Novel Homeobox Gene Hesx1

Discussion

Member takes

Implication

Key Points

Cite This Study

Sequence, Genomic Organization, and Expression of the Novel Homeobox Gene Hesx1

Discussion

Member takes

Implication

Key Points

Cite This Study

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