We report a new reversible fluorescent two-photon (TP) probe (PY-CA) with high TP absorption cross section and pH-independent fluorescence response, which allow monitoring of O2˙(-) fluxes dynamically, selectively and sensitively. The imaging results indicate that O2˙(-) at high levels can shorten the life of Caenorhabditis elegans.
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Zhang et al. (2015) studied this question.
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