What question did this study set out to answer?

To isolate and characterize the distinct phospholipases produced by Bacillus cereus and evaluate their biochemical actions on erythrocytes, bone, and kidney tissues.

July 1, 1965Journal of BacteriologyOpen Access

Characterization of the Phospholipases ofBacillus cereusand Their Effects on Erythrocytes, Bone, and Kidney Cells

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Authors

MSMilton W. SleinWashington University in St. Louis GLGerald F. LoganUnited States Army Medical Research Institute of Infectious Diseases

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Implication

In vitro study characterizes distinct phospholipases from Bacillus cereus affecting kidney, bone, and red blood cells, indicating diverse mechanisms of bacterial cytotoxicity.

Key Points

To isolate and characterize the distinct phospholipases produced by Bacillus cereus and evaluate their biochemical actions on erythrocytes, bone, and kidney tissues.
Fractionated Bacillus cereus culture filtrates using DEAE-cellulose column chromatography and analyzed phospholipid cleavage products via quantitative paper chromatography.
Measured phosphatasemia factor activity via the release of alkaline phosphatase from kidney cortex homogenates and tested hemolytic capacity against erythrocytes in the presence and absence of inhibitors.
Identified distinct phospholipase entities hydrolyzing phosphatidyl inositol, sphingomyelin, and phosphatidyl choline or ethanolamine, displaying differential susceptibility to trypsin inactivation.
Observed that the highest phosphatasemia factor activity co-purifies with phosphatidyl inositol-phospholipase, while crude soybean lecithin inhibits both phosphatasemia factor and hemolysin activities.

Cite This Study

Slein et al. (1965) studied this question.

synapsesocial.com/papers/6a7013418031ec7bb1dc1874 https://doi.org/10.1128/jb.90.1.69-81.1965

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Implication

In vitro study characterizes distinct phospholipases from Bacillus cereus affecting kidney, bone, and red blood cells, indicating diverse mechanisms of bacterial cytotoxicity.

Characterization of the Phospholipases ofBacillus cereusand Their Effects on Erythrocytes, Bone, and Kidney Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Characterization of the Phospholipases ofBacillus cereusand Their Effects on Erythrocytes, Bone, and Kidney Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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