Mechanistic study reveals that centromeric protein interactions drive replication origin clustering in Agrobacterium tumefaciens, indicating their vital role in preserving multipartite genomes.
About 10% of sequenced bacteria have multiple replicons, also known as multipartite genomes. How these multipartite genomes are maintained is still poorly understood. Here, we use Agrobacterium tumefaciens as a model and show that the replication origins of the four replicons are clustered through direct interactions between the centromeric proteins; disruption of origin clustering leads to the loss of replicons. Thus, our study provided evidence that centromeric clustering is important for maintaining multipartite genomes.
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Ren et al. (2022) studied this question.
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