What question did this study set out to answer?

To identify the specific retroviral genetic determinants that regulate the lytic and leukemogenic properties of the Friend murine leukemia virus.

July 1, 1991Proceedings of the National Academy of SciencesOpen Access

Substitution of leucine for isoleucine in a sequence highly conserved among retroviral envelope surface glycoproteins attenuates the lytic effect of the Friend murine leukemia virus.

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Authors

MSMarc SitbonCACI International (United States)LDL d'AuriolHôpital Robert-Debré HEHeinz EllerbrokRobert Koch Institute

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Implication

Experimental study demonstrates that a single envelope substitution attenuates Friend murine leukemia virus virulence in newborn mice, highlighting a critical conserved retroviral domain.

Key Points

To identify the specific retroviral genetic determinants that regulate the lytic and leukemogenic properties of the Friend murine leukemia virus.
Generated chimeric infectious viral constructs and site-directed point mutations between a virulent Friend murine leukemia virus strain and an attenuated variant.
Inoculated newborn mice to evaluate viral particle budding in blood reticulocytes and monitor subsequent development of severe hemolytic anemia and erythroleukemia.
Severe early hemolytic anemia consistently correlated with increased quantities of circulating reticulocytes bearing budding retroviral particles.
A single leucine-to-isoleucine substitution at position 348 within a universally conserved five-amino-acid motif of the extracellular SU envelope glycoprotein was sufficient to attenuate lytic pathology.
Deletion of a second repeat in the transcriptional enhancer region of the viral long terminal repeats further diminished both lytic and leukemogenic effects.

Cite This Study

Sitbon et al. (1991) studied this question.

synapsesocial.com/papers/6a701ad6e5469ee92be0deb0 https://doi.org/10.1073/pnas.88.13.5932

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Implication

Experimental study demonstrates that a single envelope substitution attenuates Friend murine leukemia virus virulence in newborn mice, highlighting a critical conserved retroviral domain.

Substitution of leucine for isoleucine in a sequence highly conserved among retroviral envelope surface glycoproteins attenuates the lytic effect of the Friend murine leukemia virus.

Discussion

Member takes

Implication

Key Points

Cite This Study

Substitution of leucine for isoleucine in a sequence highly conserved among retroviral envelope surface glycoproteins attenuates the lytic effect of the Friend murine leukemia virus.

Discussion

Member takes

Implication

Key Points

Cite This Study

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