What question did this study set out to answer?

The aim is to characterize the electrical properties of single bullfrog atrial cells to enhance understanding of their functioning.

July 1, 1981The Journal of General PhysiologyOpen Access

Active and passive electrical properties of single bullfrog atrial cells.

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Population

Single cells from the bullfrog (Rana catesbeiana) atrium

Design

Preclinical

Authors

JHJoseph R. HumeElectrophysiology WGWayne R. GilesQB3

Discussion

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Implication

Establishes isolated bullfrog atrial cells as a model for voltage-clamp studies; leaves open translation to mammalian cardiac electrophysiology.

Key Points

The aim is to characterize the electrical properties of single bullfrog atrial cells to enhance understanding of their functioning.
Single bullfrog atrial cells were prepared using enzymatic dispersion for analysis.
Electrophysiological measurements were performed in normal Ringer's solution to assess action potentials.
A bridge circuit was employed to evaluate the cells' input resistance and time constants.
Stable resting potential of approximately -88 mV was recorded in the cells.
Action potentials reached up to 125 mV with a duration of about 720 ms.
Calculated electrical constants included input resistance of 220 M omega and time constant of 20 ms.

Structured PICO

Population

Single cells from the bullfrog (Rana catesbeiana) atrium

Intervention

Electrophysiological measurements including exposure to tetrodotoxin (10(-6) g/ml) and verapamil (3 x 10(-6) M)

Outcome

Active and passive electrical properties (resting potential, action potential characteristics, electrical constants)surrogate

Single bullfrog atrial cells demonstrate stable electrical properties and morphology that make them a suitable preparation for voltage-clamp studies.

Cite This Study

Hume et al. (1981) studied this question.

synapsesocial.com/papers/6a701ee55d37378ac1dd2ef7 https://doi.org/10.1085/jgp.78.1.19

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Implication

Establishes isolated bullfrog atrial cells as a model for voltage-clamp studies; leaves open translation to mammalian cardiac electrophysiology.

Active and passive electrical properties of single bullfrog atrial cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Active and passive electrical properties of single bullfrog atrial cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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