What question did this study set out to answer?

To identify novel variable-number tandem repeat (VNTR) PCR targets across the Mycobacterium tuberculosis complex and assess their ability to distinguish bacterial strains for molecular epidemiology.

February 1, 2002Microbiology

Discrimination of Mycobacterium tuberculosis complex bacteria using novel VNTR-PCR targets

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Authors

RSRobin SkuceAgri Food and Biosciences Institute TMT. McCorryQueen's University Belfast JMJ. McCarrollDepartment of Agriculture and Rural Development

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Implication

Genotyping analysis demonstrates high-resolution discrimination of Mycobacterium bovis isolates using novel VNTR-PCR targets, suggesting improved tools for tuberculosis surveillance.

Key Points

To identify novel variable-number tandem repeat (VNTR) PCR targets across the Mycobacterium tuberculosis complex and assess their ability to distinguish bacterial strains for molecular epidemiology.
Identified seven novel VNTR loci in the Mycobacterium tuberculosis H37Rv genome.
Evaluated six of these VNTR targets across a panel of 100 distinct Mycobacterium bovis isolates and compared performance with spoligotyping and exact tandem repeat (ETR) typing.
Novel VNTR loci exhibited three to seven alleles per locus, identifying 33 distinct allele profiles compared to 29 for spoligotyping and 22 for ETR typing.
Combining novel VNTR- and ETR-typing resolved 51 distinct strain profiles across the 100 M. bovis isolates.
Identified VNTRs were mapped to intergenic regions and open reading frames, including glycine-asparagine-rich PPE protein genes.

Cite This Study

Skuce et al. (2002) studied this question.

synapsesocial.com/papers/6a70268c5671bbf00bc2a898 https://doi.org/10.1099/00221287-148-2-519

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Implication

Genotyping analysis demonstrates high-resolution discrimination of Mycobacterium bovis isolates using novel VNTR-PCR targets, suggesting improved tools for tuberculosis surveillance.

Discrimination of Mycobacterium tuberculosis complex bacteria using novel VNTR-PCR targets

Discussion

Member takes

Implication

Key Points

Cite This Study

Discrimination of Mycobacterium tuberculosis complex bacteria using novel VNTR-PCR targets

Discussion

Member takes

Implication

Key Points

Cite This Study

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