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Determine the reversibility and ionic mechanisms responsible for acetylcholine-induced electrical uncoupling in pancreatic acinar cells.

January 1, 1978The Journal of PhysiologyOpen Access

Pancreatic acinar cells: acetylcholine‐evoked electrical uncoupling and its ionic dependency

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Authors

NINaofumi IwatsukiTohoku University OPOle H. PetersenUniversity of Geneva

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Implication

Experimental study demonstrates acetylcholine-evoked electrical uncoupling in rodent pancreatic acinar cells, indicating transient and sustained stimulation rely on distinct calcium sources.

Key Points

Determine the reversibility and ionic mechanisms responsible for acetylcholine-induced electrical uncoupling in pancreatic acinar cells.
Inserted up to three intracellular microelectrodes into adjacent acinar cells of isolated mouse or rat pancreas preparations to inject current and monitor cell-to-cell electrical coupling.
Delivered acetylcholine by micro-iontophoresis while altering the extracellular superfusion fluid with calcium-free EGTA solutions and divalent cations such as manganese, nickel, and cobalt.
Brief 5- to 10-second pulses of acetylcholine caused rapid, fully reversible electrical uncoupling between neighboring acinar cells that persisted even during 50-minute exposures to calcium-free solutions.
Manganese gradually abolished uncoupling, while nickel and cobalt enhanced transient uncoupling but immediately and reversibly recoupled cells during sustained acetylcholine exposure.
Internal calcium stores trigger uncoupling from short acetylcholine pulses, whereas continuous extracellular calcium influx sustains prolonged uncoupling.

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Iwatsuki et al. (1978) studied this question.

synapsesocial.com/papers/6a70277d35aa2c282ce17546 https://doi.org/10.1113/jphysiol.1978.sp012135

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Implication

Experimental study demonstrates acetylcholine-evoked electrical uncoupling in rodent pancreatic acinar cells, indicating transient and sustained stimulation rely on distinct calcium sources.

Pancreatic acinar cells: acetylcholine‐evoked electrical uncoupling and its ionic dependency

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Implication

Key Points

Cite This Study

Pancreatic acinar cells: acetylcholine‐evoked electrical uncoupling and its ionic dependency

Discussion

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Implication

Key Points

Cite This Study

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