What question did this study set out to answer?

To identify and characterize the role of the mitogen-activated protein kinase gene fmk1 in root penetration and pathogenesis in the fungal wilt pathogen Fusarium oxysporum.

March 1, 2001Molecular MicrobiologyOpen Access

A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis

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Authors

APAntonio Di PietroCordoba University FGFé I. Garcı́a-MaceiraIRIS Technology Solutions (Spain)EMEmese MegléczAix-Marseille Université

Discussion

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Implication

Functional genetic study demonstrates that FMK1 MAP kinase controls root penetration and virulence in Fusarium oxysporum, indicating a conserved role across plant pathogens.

Key Points

To identify and characterize the role of the mitogen-activated protein kinase gene fmk1 in root penetration and pathogenesis in the fungal wilt pathogen Fusarium oxysporum.
Generated targeted fmk1 gene inactivation mutants in Fusarium oxysporum f. sp. lycopersici.
Evaluated vegetative growth, surface hydrophobicity, cell wall-degrading enzyme (pectate lyase pl1) expression, and virulence on tomato plants and fruit.
Tested cross-species functional complementation using the orthologous MAPK gene Pmk1 from the foliar rice pathogen Magnaporthe grisea.
Inactivated fmk1 mutants completely lost pathogenicity on tomato plants, failed to form penetration hyphae, and showed impaired root attachment and invasive growth, despite displaying normal vegetative growth and conidiation.
Mutants exhibited reduced surface hydrophobicity and drastically decreased transcript levels of the pectate lyase gene pl1.
Expression of the Magnaporthe grisea ortholog Pmk1 complemented invasive growth defects and partially restored root attachment, hydrophobicity, and pathogenicity in fmk1 mutants.

Cite This Study

Pietro et al. (2001) studied this question.

synapsesocial.com/papers/6a702b1da528af2d65c35f14 https://doi.org/10.1111/j.1365-2958.2001.02307.x

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Implication

Functional genetic study demonstrates that FMK1 MAP kinase controls root penetration and virulence in Fusarium oxysporum, indicating a conserved role across plant pathogens.

A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis

Discussion

Member takes

Implication

Key Points

Cite This Study

A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis

Discussion

Member takes

Implication

Key Points

Cite This Study

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