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September 1, 1994Journal of Biological ChemistryOpen Access

Requirement of amino-terminal modification for striated muscle alpha-tropomyosin function.

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Population

Recombinant chicken striated muscle alpha-tropomyosin expressed in insect Sf9 cells and E. coli

Comparison

Acetylated recombinant tropomyosin and… vs Chicken striated alpha-tropomyosin purified from…

Design

Preclinical

Authors

MUM Urbančı́kováSlovak Medical UniversitySHSarah E. Hitchcock‐DeGregoriRutgers, The State University of New Jersey

Discussion

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Implication

Acetylation required for normal tropomyosin-actin binding; leaves open in vivo relevance to cardiac or skeletal muscle disorders.

Structured PICO

P
Population
Recombinant chicken striated muscle alpha-tropomyosin expressed in insect Sf9 cells and E. coli
I
Intervention
Acetylated recombinant tropomyosin and unacetylated short fusion recombinant tropomyosin (f3-tropomyosin with AlaSer-Arg on NH2 terminus)
C
Comparator
Chicken striated alpha-tropomyosin purified from muscle
O
Outcome
Actin affinity and polymerization propertiessurrogate

NH2-terminal acetylation is essential for the normal function and cooperative binding of striated muscle alpha-tropomyosin to actin.

Cite This Study

Urbančı́ková et al. (1994) studied this question.

synapsesocial.com/papers/6a702ce7b27f158178273adchttps://doi.org/10.1016/s0021-9258(19)51083-3
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Properties of Non-polymerizable Tropomyosin Obtained by Carboxypeptidase A Digestion1976 · 42 citations
  2. 2The amino terminus of muscle tropomyosin is a major determinant for function.1990 · 105 citations
  3. 3Polymerizability of rabbit skeletal tropomyosin: effects of enzymic and chemical modifications1977 · 101 citations
  4. 4Unfolding domains of recombinant fusion αα‐tropomyosin1992 · 32 citations
  5. 5In vitro and in vivo characterization of four fibroblast tropomyosins produced in bacteria: TM-2, TM-3, TM-5a, and TM-5b are co-localized in interphase fibroblasts.1992 · 73 citations