April 15, 1994The Journal of Cell BiologyOpen Access

Overexpression of human fibroblast caldesmon fragment containing actin-, Ca++/calmodulin-, and tropomyosin-binding domains stabilizes endogenous tropomyosin and microfilaments.

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Population

CHO cells (stable transfectants)

Comparison

Expression of CaD39 vs Stable CHO clones expressing CaD40

Design

Preclinical

Authors

KWKerri S. WarrenUniversity of Iowa JLJenny LinEmory University DWDawn D. WamboldtUniversity of Iowa

Discussion

Member takes

Implication

May guide cytoskeletal studies in vascular cells; leaves open relevance to cardiac function or disease.

Structured PICO

Population

CHO cells (stable transfectants)

Intervention

Expression of CaD39 (COOH-terminal fragment of human caldesmon, aa.244-538)

Comparator

Stable CHO clones expressing CaD40 (NH2-terminal fragment, aa.1-243)

Outcome

Stability of actin filaments and endogenous tropomyosin (TM)surrogate

Expression of the COOH-terminal fragment of human caldesmon (CaD39) enhances tropomyosin binding to F-actin in vivo, stabilizing actin microfilament bundles.

Cite This Study

Warren et al. (1994) studied this question.

synapsesocial.com/papers/6a702ce7b27f158178273add https://doi.org/10.1083/jcb.125.2.359

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Overexpression of human fibroblast caldesmon fragment containing actin-, Ca++/calmodulin-, and tropomyosin-binding domains stabilizes endogenous tropomyosin and microfilaments.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Overexpression of human fibroblast caldesmon fragment containing actin-, Ca++/calmodulin-, and tropomyosin-binding domains stabilizes endogenous tropomyosin and microfilaments.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider