Phospholipid Metabolism in Cultured Neuroblastoma and Glioma Cells Incubated With Carbamylcholine and Norepinephrine

Cultures of cloned neuroblastoma cells (N1E) in stationary phase and cloned glioma cells (C2 1 ) in confluency showed substantial differences in phospholipid composition. As a percentage of lipid P, N1E contained more phosphatidylcholine, less ethanolamine phosphoglycerides and much less sphingomyelin than C2 1 . When incubated with 32 P i both cell lines incorporated comparable amounts of radioactivity into total phospholipids. In NIE, phosphatidylcholine contained much more and phosphatidylinositol and phosphatidic acid somewhat less label as compared to C2 1 . The presence in the incubation medium of either norepinephrine or carbamylcholine failed to elicit stimulation of 32 P incorporation into any phospholipid class.