What question did this study set out to answer?

To determine the biochemical factors and mechanisms controlling the dissociation of protein kinase C from plasma membranes.

December 1, 1985Journal of Biological ChemistryOpen Access

Interaction of protein kinase C with membranes is regulated by Ca2+, phorbol esters, and ATP.

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To determine the biochemical factors and mechanisms controlling the dissociation of protein kinase C from plasma membranes.
Measured dissociation kinetics of protein kinase C from isolated plasma membranes across varying calcium concentrations ranging from 0.1 to 1 µM.
Evaluated the membrane-stabilizing effects of 4-beta-phorbol 12,13-dibutyrate and tested reversal by ATP, magnesium chloride, and nonhydrolyzable nucleotide analogues.
In the absence of phorbol esters, protein kinase C dissociation is rapid upon calcium depletion between 0.1 and 1 µM, whereas 4-beta-phorbol 12,13-dibutyrate stabilizes membrane binding by reducing the calcium Km to approximately 15 nM.
ATP reverses phorbol-ester-mediated membrane stabilization with an apparent nucleotide Km of 6.5 µM in the presence of magnesium chloride, shifting the calcium Km to approximately 760 nM.
Calcium-dependent enzyme dissociation exhibits strong negative cooperativity, consistent with multiple interacting calcium-binding sites on protein kinase C.

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Implication

In vitro assay reveals how calcium, phorbol esters, and ATP modulate protein kinase C membrane dissociation, indicating a phosphorylation-driven regulatory mechanism.

Cite This Study

Wolf et al. (1985) studied this question.

synapsesocial.com/papers/6a7031cf26a7f98052dcb335 https://doi.org/10.1016/s0021-9258(17)36318-4

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Implication

In vitro assay reveals how calcium, phorbol esters, and ATP modulate protein kinase C membrane dissociation, indicating a phosphorylation-driven regulatory mechanism.

Interaction of protein kinase C with membranes is regulated by Ca2+, phorbol esters, and ATP.

Key Points

Discussion

Implication

Cite This Study

Interaction of protein kinase C with membranes is regulated by Ca2+, phorbol esters, and ATP.

Key Points

Discussion

Implication

Cite This Study

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