What question did this study set out to answer?

To assess whether peri-Golgi vesicles transport anterograde cargo or recycle resident proteins in order to distinguish between competing models of intra-Golgi trafficking.

December 17, 2001The Journal of Cell BiologyOpen Access

Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport

View Full Paper

Ask AI

Bookmark

Authors

JMJosé A. Martínez‐MenárguezUniversidad de Murcia RPRytis PrekerisUniversity of Colorado Anschutz Medical Campus VOViola OorschotGuy's Hospital

Discussion

Member takes

Implication

Immuno-electron microscopy reveals retrograde proteins but not anterograde cargo in peri-Golgi vesicles of rat cells, supporting the cisternal progression model of transport.

Key Points

To assess whether peri-Golgi vesicles transport anterograde cargo or recycle resident proteins in order to distinguish between competing models of intra-Golgi trafficking.
Performed quantitative immunoelectron microscopy on normal rat kidney (NRK) cells to map protein content in peri-Golgi vesicles and cisternal rims.
Tracked localization of Golgi resident enzyme mannosidase II, transport machinery (giantin, KDEL-receptor, rBet1), and the anterograde marker vesicular stomatitis virus protein G.
Coatomer protein I-coated cisternal rims and peri-Golgi vesicles contained significant levels of mannosidase II, giantin, KDEL-receptor, and rBet1.
The anterograde cargo marker vesicular stomatitis virus protein G was largely absent from peri-Golgi vesicles.

Cite This Study

Martínez‐Menárguez et al. (2001) studied this question.

synapsesocial.com/papers/6a703525a2d7cf2e39c2a26a https://doi.org/10.1083/jcb.200108029

View Full Paper

Ask AI

Bookmark

Implication

Immuno-electron microscopy reveals retrograde proteins but not anterograde cargo in peri-Golgi vesicles of rat cells, supporting the cisternal progression model of transport.

Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport

Discussion

Member takes

Implication

Key Points

Cite This Study

Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider