Physical mapping analysis uncovers local DNA domain amplifications across human Yq11, highlighting complex repetitive genomic architecture in male sex chromosomes.
Key Points
Characterize the complex genomic architecture of the euchromatic long arm of the human Y chromosome (Yq11) by overcoming limitations caused by repetitive sequences and high DNA methylation.
Screened 67 yeast artificial chromosome (YAC) clones isolated from three CEPH libraries.
Performed quantitative blot hybridization targeting 51 distinct DNA loci.
Constructed a 13 Mb contiguous physical map spanning proximal to distal Yq11 using interval mapping.
Assembled an ordered 13 Mb physical contig encompassing the euchromatic Yq11 interval.
Identified localized genomic amplification events across multiple distinct DNA domains in distal Yq11.
Established a structural model outlining the spatial arrangement and repetitive organization of the region.