What question did this study set out to answer?

To compare MIB-1 monoclonal antibody staining against established cell proliferation markers and determine optimal cutoff points for classifying breast carcinoma proliferation rates.

April 15, 2002Cancer

Correlation between MIB‐1 and other proliferation markers

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Authors

FSFrédérique SpyratosHôpital René Huguenin MFM. Ferrero-PoüsHôpital René Huguenin MTMartine TrassardUniversité de Bordeaux

Discussion

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Implication

Observational study demonstrates strong correlations between MIB-1 and standard proliferation markers in breast carcinomas, suggesting threshold selection depends on clinical goals.

Key Points

To compare MIB-1 monoclonal antibody staining against established cell proliferation markers and determine optimal cutoff points for classifying breast carcinoma proliferation rates.
Evaluated 185 invasive breast carcinomas for cell proliferation using MIB-1 monoclonal antibody staining and histological parameters (Scarff-Bloom-Richardson grade, mitotic index) on paraffin sections.
Assessed S-phase fraction (SPF) via flow cytometry and thymidine-kinase (TK) activity on frozen tissue samples.
Conducted multivariate analyses testing five MIB-1 threshold cutoffs (10%, 15%, 17%, 20%, and 25%) against the other proliferation metrics.
MIB-1 positivity strongly correlated with SPF, TK levels, histologic grade, and mitotic index (P = 0.0001).
Multivariate analysis showed an optimal 25% MIB-1 cutoff for distinguishing highly proliferative tumors, with mitotic index emerging as the strongest discriminating variable.
A lower MIB-1 cutoff of 10% minimized misclassification of low-proliferation tumors, whereas a 25% cutoff effectively captured rapid proliferators.

Cite This Study

Spyratos et al. (2002) studied this question.

synapsesocial.com/papers/6a703991439bab0cabc3131d https://doi.org/10.1002/cncr.10458

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April 15, 2002Cancer

Correlation between MIB‐1 and other proliferation markers

View Full Paper

Ask AI

Bookmark

Authors

FSFrédérique SpyratosHôpital René Huguenin MFM. Ferrero-PoüsHôpital René Huguenin MTMartine TrassardUniversité de Bordeaux

Discussion

Member takes

Implication

Observational study demonstrates strong correlations between MIB-1 and standard proliferation markers in breast carcinomas, suggesting threshold selection depends on clinical goals.

Key Points

To compare MIB-1 monoclonal antibody staining against established cell proliferation markers and determine optimal cutoff points for classifying breast carcinoma proliferation rates.
Evaluated 185 invasive breast carcinomas for cell proliferation using MIB-1 monoclonal antibody staining and histological parameters (Scarff-Bloom-Richardson grade, mitotic index) on paraffin sections.
Assessed S-phase fraction (SPF) via flow cytometry and thymidine-kinase (TK) activity on frozen tissue samples.
Conducted multivariate analyses testing five MIB-1 threshold cutoffs (10%, 15%, 17%, 20%, and 25%) against the other proliferation metrics.
MIB-1 positivity strongly correlated with SPF, TK levels, histologic grade, and mitotic index (P = 0.0001).
Multivariate analysis showed an optimal 25% MIB-1 cutoff for distinguishing highly proliferative tumors, with mitotic index emerging as the strongest discriminating variable.
A lower MIB-1 cutoff of 10% minimized misclassification of low-proliferation tumors, whereas a 25% cutoff effectively captured rapid proliferators.

Cite This Study

Spyratos et al. (2002) studied this question.

synapsesocial.com/papers/6a703991439bab0cabc3131d https://doi.org/10.1002/cncr.10458

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