What question did this study set out to answer?

To elucidate the function of the transcription factor IscR and determine whether its Fe-S cluster mediates the regulation of Fe-S cluster assembly genes in Escherichia coli.

December 11, 2001Proceedings of the National Academy of SciencesOpen Access

IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins

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Authors

CSChristopher SchwartzDigital Research Alliance of Canada JGJennifer L. GielScientific Solutions (United States)TPThomas PatschkowskiBielefeld University

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Implication

Molecular analysis reveals that IscR represses iron-sulfur cluster assembly operon expression in Escherichia coli, indicating a homeostatic autoregulatory feedback loop.

Key Points

To elucidate the function of the transcription factor IscR and determine whether its Fe-S cluster mediates the regulation of Fe-S cluster assembly genes in Escherichia coli.
Measured iscRSUA operon expression in wild-type, iscR-deletion, and Fe-S cluster assembly-deficient (iscS and hscA null) Escherichia coli strains.
Performed in vitro transcription assays to assess direct IscR-mediated repression of the iscR promoter.
Examined anaerobically purified IscR using electron paramagnetic resonance (EPR) spectroscopy to identify associated prosthetic groups.
Deletion of iscR increased expression of the iscRSUA operon, and in vitro transcription confirmed direct repression of the iscR promoter by IscR.
Electron paramagnetic resonance spectroscopy demonstrated that anaerobically isolated IscR coordinates a [2Fe-2S](1+) cluster.
Repression of iscR was substantially diminished in iscS or hscA null mutant backgrounds with defective Fe-S cluster assembly.

Cite This Study

Schwartz et al. (2001) studied this question.

synapsesocial.com/papers/6a7044762fdcb5703edb582e https://doi.org/10.1073/pnas.251550898

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Implication

Molecular analysis reveals that IscR represses iron-sulfur cluster assembly operon expression in Escherichia coli, indicating a homeostatic autoregulatory feedback loop.

IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins

Discussion

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Implication

Key Points

Cite This Study

IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins

Discussion

Member takes

Implication

Key Points

Cite This Study

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