What question did this study set out to answer?

To investigate the roles of membrane internalization and vesicle fusion in neutrophil adhesion in response to lipopolysaccharide (LPS) and TNF.

December 1, 1996The Journal of ImmunologyOpen Access

Potential role of membrane internalization and vesicle fusion in adhesion of neutrophils in response to lipopolysaccharide and TNF

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Randomized trial shows the requirement of vesicle fusion for neutrophil adhesion in response to LPS and TNF, suggesting important signaling pathways.

To investigate the roles of membrane internalization and vesicle fusion in neutrophil adhesion in response to lipopolysaccharide (LPS) and TNF.
Human polymorphonuclear leukocytes (PMNs) were treated with fluorescently labeled monomeric LPS and soluble CD14.
Adhesion responses were analyzed over time, including temperature and pharmacological inhibition (cytochalasin D, wortmannin).
The effects of fNLLP and LPS on adhesion were compared to evaluate differences in signaling requirements.
Adhesion to LPS required a lag period of 15-20 minutes, consistent with internalization and vesicle fusion processes.
Adhesion was blocked at 19 degrees C, indicating the necessity of vesicle fusion for LPS signaling.
Both cytochalasin D and wortmannin inhibited LPS-induced adhesion, suggesting the importance of endocytosis and early endosomal events.

Detmers et al. (1996) studied this question.

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Randomized trial shows the requirement of vesicle fusion for neutrophil adhesion in response to LPS and TNF, suggesting important signaling pathways.