What question did this study set out to answer?

To isolate microparticles from platelet-free plasma and characterize their biochemical composition, ultrastructure, and contractile protein content.

July 1, 1971British Journal of Haematology

The Presence of Contractile Proteins in Platelet Microparticles Isolated from Human and Animal Platelet‐free Plasma

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Authors

NCN. CrawfordAstraZeneca (United Kingdom)

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Implication

Laboratory study demonstrates functional contractile proteins in human and animal platelet microparticles, suggesting an origin from platelet membrane budding.

Key Points

To isolate microparticles from platelet-free plasma and characterize their biochemical composition, ultrastructure, and contractile protein content.
Isolated microparticles from human and animal platelet-free plasma using plasma dilution to lower specific gravity followed by high-speed ultracentrifugation.
Quantified protein, cholesterol, and phospholipid content, comparing values with whole platelet homogenates and sucrose density gradient subcellular fractions.
Assayed ATPase enzymatic activity and evaluated antigenic cross-reactivity with thrombosthenin antisera to determine inhibition of clot retraction and membrane binding.
Microparticles exhibited ATPase activity with characteristics matching platelet thrombosthenin in response to divalent cations, mersalyl, and ouabain, despite a lower specific activity.
Antisera against microparticles and thrombosthenin both inhibited in vitro clot retraction at high dilution and inhibited the Mg2+ ATPase activity of platelet homogenates.
Antisera against both targets reacted with low-density membrane fragments on sucrose gradients, indicating particles originate via platelet surface budding or cytoplasmic herniation.

Cite This Study

N. Crawford (1971) studied this question.

synapsesocial.com/papers/6a704c0aa528af2d65c371c1 https://doi.org/10.1111/j.1365-2141.1971.tb03416.x

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Implication

Laboratory study demonstrates functional contractile proteins in human and animal platelet microparticles, suggesting an origin from platelet membrane budding.

The Presence of Contractile Proteins in Platelet Microparticles Isolated from Human and Animal Platelet‐free Plasma

Discussion

Member takes

Implication

Key Points

Cite This Study

The Presence of Contractile Proteins in Platelet Microparticles Isolated from Human and Animal Platelet‐free Plasma

Discussion

Member takes

Implication

Key Points

Cite This Study

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