What question did this study set out to answer?

Investigate cell wall glucan alterations and evaluate the functional necessity of increased chitin synthesis in Saccharomyces cerevisiae mutants lacking the GGP1/GAS1 gene.

January 1, 1997Journal of BacteriologyOpen Access

Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1delta mutant of Saccharomyces cerevisiae

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Authors

LPLaura PopoloUniversity of Milan DGD GilardelliUniversity of Milan PBPaola BonfanteJohn Innes Centre

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Implication

Genetic analysis reveals elevated chitin compensates for cell wall glucan assembly defects in yeast mutants, indicating an essential structural rescue mechanism.

Key Points

Investigate cell wall glucan alterations and evaluate the functional necessity of increased chitin synthesis in Saccharomyces cerevisiae mutants lacking the GGP1/GAS1 gene.
Measured alkali-soluble and alkali-insoluble beta-glucan fractions in wild-type, ggp1delta, and chs3delta ggp1delta double mutant yeast strains.
Assessed cell viability and wall morphology using electron microscopy, the chitin synthase inhibitor nikkomycin Z, and genetic interaction crosses with kre6delta and pkc1delta.
In ggp1delta cells, alkali-insoluble 1,6-beta-D-glucan levels dropped to 50% of wild-type levels, and the ratio of alkali-soluble to alkali-insoluble glucan approximately doubled.
Abolishing compensatory chitin synthesis in chs3delta ggp1delta mutants led to severe reductions in growth rate, loss of normal cell wall ultrastructure, and reduced alkali-insoluble glucan levels to 57% of wild type.
Pharmacological inhibition with nikkomycin Z produced hypersensitivity in ggp1delta cells, while double deletions with kre6delta and pkc1delta resulted in synthetic lethality.

Cite This Study

Popolo et al. (1997) studied this question.

synapsesocial.com/papers/6a704e8dce524a4339c40e88 https://doi.org/10.1128/jb.179.2.463-469.1997

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Implication

Genetic analysis reveals elevated chitin compensates for cell wall glucan assembly defects in yeast mutants, indicating an essential structural rescue mechanism.

Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1delta mutant of Saccharomyces cerevisiae

Discussion

Member takes

Implication

Key Points

Cite This Study

Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1delta mutant of Saccharomyces cerevisiae

Discussion

Member takes

Implication

Key Points

Cite This Study

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