What question did this study set out to answer?

To establish and evaluate a rapid microtiter menadione-augmented MTT reduction assay for measuring antifungal susceptibility in Candida albicans and Aspergillus fumigatus.

March 1, 1995Journal of Clinical MicrobiologyOpen Access

Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay

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Authors

BJBernhard JahnGoethe University Frankfurt EME MartínJohannes Gutenberg University Mainz ASA StuebenInstitute of Medical Microbiology and Hygiene

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Implication

In vitro study demonstrates reliable antifungal susceptibility profiling in Candida albicans and Aspergillus fumigatus, indicating practical utility for clinical diagnostic testing.

Key Points

To establish and evaluate a rapid microtiter menadione-augmented MTT reduction assay for measuring antifungal susceptibility in Candida albicans and Aspergillus fumigatus.
Inoculated C. albicans or conidial suspensions of A. fumigatus into microtiter plates, allowing Aspergillus hyphal outgrowth for 12 to 14 hours at 30°C.
Treated fungal cultures with amphotericin B, fluconazole, or itraconazole for 24 hours, followed by a 3-hour incubation with MTT and 0.1 mM menadione.
Quantified formazan photometrically after acid isopropanol extraction and compared results with standard macrodilution minimum inhibitory concentration determinations.
Produced well-defined dose-response curves directly reflecting antifungal-mediated impairment of fungal mitochondrial respiration.
Showed excellent qualitative correlation between MTT assay results and standard macrodilution MIC determinations for C. albicans.
Demonstrated that fluconazole fails to exert fungistatic action against a sensitive C. albicans strain in the presence of serum.

Cite This Study

Jahn et al. (1995) studied this question.

synapsesocial.com/papers/6a704f0ba2d7cf2e39c2a584 https://doi.org/10.1128/jcm.33.3.661-667.1995

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Implication

In vitro study demonstrates reliable antifungal susceptibility profiling in Candida albicans and Aspergillus fumigatus, indicating practical utility for clinical diagnostic testing.

Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay

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Implication

Key Points

Cite This Study

Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay

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Implication

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