What question did this study set out to answer?

Investigate the enzymatic synthesis of water-insoluble glucans from UDP-d-glucose using enzyme fractions derived from Phaseolus aureus and Lupinus albus.

October 1, 1968PLANT PHYSIOLOGYOpen Access

Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus

View Full Paper

Ask AI

Bookmark

Authors

HFHarold M. FlowersChildren's Hospital of Philadelphia KBK. K. BatraAsher Consulting (United States)JKJennifer KempErasmus MC

Discussion

Member takes

Implication

In vitro biochemical study reveals synthesis of beta-1,3-glucans from UDP-glucose in seedling extracts, indicating exclusive formation of specific linkages without cellulose bonds.

Key Points

Investigate the enzymatic synthesis of water-insoluble glucans from UDP-d-glucose using enzyme fractions derived from Phaseolus aureus and Lupinus albus.
Extracted particulate and digitonin-solubilized enzyme fractions (34,000g pellet and supernatant) from Phaseolus aureus and Lupinus albus tissues.
Assayed glucan biosynthesis using radioactive UDP-d-glucose-(14)C across various substrate concentrations.
Analyzed the chemical solubility and degradation products of the resulting polymers to identify specific interglucosidic linkage types.
Digitonin treatment substantially increased specific enzymatic activity per unit of protein in both the 34,000g pellet and the supernatant liquid relative to untreated particles.
Substrate incorporation reached at least 50% at an optimal UDP-d-glucose concentration of 10⁻³ M, producing polymers that were 90% to 95% soluble in hot, dilute alkali.
Degradation products established that the synthesized polymers were exclusively β-(1→3)-glucans, with no measurable β-(1→4)-d-glucose linkages detected.

Cite This Study

Flowers et al. (1968) studied this question.

synapsesocial.com/papers/6a704f8675498292b709b8bf https://doi.org/10.1104/pp.43.10.1703

View Full Paper

Ask AI

Bookmark

Implication

In vitro biochemical study reveals synthesis of beta-1,3-glucans from UDP-glucose in seedling extracts, indicating exclusive formation of specific linkages without cellulose bonds.

Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus

Discussion

Member takes

Implication

Key Points

Cite This Study

Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider