Robust HPV‐16 Detection Workflow for Formalin‐Fixed Cancer Tissue and Its Application for Oral Squamous Cell Carcinoma

Shizuka Morodomi; Akiyuki Hirosue; Akhinur Rahman; Kyotaro Nohata; Misaki Matsuo; Omnia Reda; Samiul Alam Rajib; Haruki Saito; Hiroki Takeda; Ryoji Yoshida; Masafumi Nakamoto; Masatoshi Hirayama; Kenta Kawahara; Mitsuyoshi Takatori; Yorihisa Orita; Hideki Nakayama; Yorifumi Satou

doi:10.1002/cam4.70544

February 1, 2025Cancer MedicineOpen Access

Robust HPV‐16 Detection Workflow for Formalin‐Fixed Cancer Tissue and Its Application for Oral Squamous Cell Carcinoma

View Full Paper

Ask AI

Bookmark

Share

Key Points

To develop and validate a reliable HPV-16 detection protocol for formalin-fixed cancer specimens that overcomes issues related to DNA fragmentation and sequence variation.
Incorporated a preliminary DNA quality assessment step alongside whole-genome multiplex PCR targeting HPV-16 to reduce false negatives from fragmented DNA and viral mutations.
Validated the assay using known HPV-16-positive oropharyngeal cancer controls, then applied the workflow across a cohort of N=112 oral squamous cell carcinoma (OSCC) samples.
Used droplet digital PCR (ddPCR) to quantify HPV-16 viral loads and performed comprehensive genomic characterization to identify viral integration sites.
Multiplex PCR yielded faint bands or smears indicating potential HPV-16 presence in 7 of 112 OSCC cases (6.25%).
Droplet digital PCR demonstrated variable HPV-16 copy levels across samples, reflecting either clonal viral origin or partial tumor positivity.
Comprehensive analysis identified genomic integration of a partially deleted HPV-16 sequence into the intronic region of the host gene TMEM94 on chromosome 17 in OSCC.

No abstract yet

We only have this paper's title and publication details.

Read it at the source

Implication

Observational cohort analysis demonstrates a robust HPV-16 detection workflow in oral squamous cell carcinoma specimens, uncovering viral genome integration into the host genome.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Morodomi et al. (2025) studied this question.

synapsesocial.com/papers/6a704ff3ac440176ef28f8ea https://doi.org/10.1002/cam4.70544

View Full Paper

Ask AI

Bookmark

Share

Robust HPV‐16 Detection Workflow for Formalin‐Fixed Cancer Tissue and Its Application for Oral Squamous Cell Carcinoma

Key Points

No abstract yet

Implication

Discussion

Cite This Study

Robust HPV‐16 Detection Workflow for Formalin‐Fixed Cancer Tissue and Its Application for Oral Squamous Cell Carcinoma

Key Points

No abstract yet

Implication

Discussion

Cite This Study

Keep reading

Keep reading