An in-house radioimmunoassay was successfully developed and validated against a commercial kit for measuring cortisol in pig serum and saliva.
Validates in-house cortisol RIA in pigs; leaves open broader applicability and salivary reliability beyond this model.
An “in-house” radioimmunoassay (RIA) procedure utilizing commercial sources of antiserum and radiolabel was developed for the simultaneous measurement of cortisol in serum and saliva from swine. A commercial RIA kit (Incstar) for cortisol was used to validate the in-house procedure for serum measurements. The correlation coefficient ( r ) between the Incstar kit and the in-house method for serum cortisol was 0.94. The Incstar kit was modified to accommodate measurement of salivary cortisol concentrations and compared to the in-house procedure. The correlation between these procedures was r = 0.81 and best described by a logarithmic (Ln) curve. Salivary cortisol measures by the in-house RIA were significantly higher than those obtained from the modified Incstar kit ( p < 0.01), a probable consequence of the different relative cross-reactions of the respective antisera to cortisone and a dilution effect on the kit standards.
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Cook et al. (1997) studied this question.
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