What question did this study set out to answer?

To identify the specific sites of DNA synthesis and resolve the fine structure and metabolic organization of deoxyribonucleoprotein (DNP) in cell nuclei.

January 1, 1963The Journal of Cell BiologyOpen Access

The Fine Structure of the DNP Component of the Nucleus

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Authors

EHElizabeth D. HayOllscoil na Gaillimhe – University of Galway JRJ P RevelCalifornia Institute of Technology

Discussion

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Implication

Electron microscopy study reveals filamentous deoxyribonucleoprotein organization in regenerating salamander cells, indicating chromatin compaction regulates DNA synthesis.

Key Points

To identify the specific sites of DNA synthesis and resolve the fine structure and metabolic organization of deoxyribonucleoprotein (DNP) in cell nuclei.
Tracked DNA synthesis in proliferating cells of regenerating salamander limbs using tritiated thymidine labeling.
Visualized ultrathin sections via electron microscope autoradiography using dilute NaOH to remove emulsion gelatin and improve resolution without displacing silver grains.
Demonstrated that nuclear DNP forms a reticular meshwork of interconnected filaments 50 to 75 Å in diameter.
Observed that the filamentous DNP meshwork disperses throughout the interphase nucleus during active DNA replication, whereas metabolically inert chromosomes and dense chromatin centers form compact, inactive aggregates.

Cite This Study

Hay et al. (1963) studied this question.

synapsesocial.com/papers/6a705bcb660549caf2c4ef40 https://doi.org/10.1083/jcb.16.1.29

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January 1, 1963The Journal of Cell BiologyOpen Access

The Fine Structure of the DNP Component of the Nucleus

View Full Paper

Ask AI

Bookmark

Authors

EHElizabeth D. HayOllscoil na Gaillimhe – University of Galway JRJ P RevelCalifornia Institute of Technology

Discussion

Member takes

Implication

Electron microscopy study reveals filamentous deoxyribonucleoprotein organization in regenerating salamander cells, indicating chromatin compaction regulates DNA synthesis.

Key Points

To identify the specific sites of DNA synthesis and resolve the fine structure and metabolic organization of deoxyribonucleoprotein (DNP) in cell nuclei.
Tracked DNA synthesis in proliferating cells of regenerating salamander limbs using tritiated thymidine labeling.
Visualized ultrathin sections via electron microscope autoradiography using dilute NaOH to remove emulsion gelatin and improve resolution without displacing silver grains.
Demonstrated that nuclear DNP forms a reticular meshwork of interconnected filaments 50 to 75 Å in diameter.
Observed that the filamentous DNP meshwork disperses throughout the interphase nucleus during active DNA replication, whereas metabolically inert chromosomes and dense chromatin centers form compact, inactive aggregates.

Cite This Study

Hay et al. (1963) studied this question.

synapsesocial.com/papers/6a705bcb660549caf2c4ef40 https://doi.org/10.1083/jcb.16.1.29

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