Unusual product ratios resulting from the gamma‐irradiation of cholesterol in liposomes

Cholesterol in aqueous suspensions of multilamellar vesicles (MLV) was exposed to gamma-irradiation (0.5-10 kGy) at 0-4 degrees C. Cholesterol oxidation products resulting from the irradiation were isolated by dry column extraction followed by preparative thin-layer chromatography (TLC) and were quantitated by on-column gas chromatography (GC). The ratio of 7-ketocholesterol/cholesterol 5,6-epoxides generated by irradiation was less than one, much lower than the ratio of ten commonly produced by autoxidation. Irradiation also produced relatively higher amounts of 7-hydroxycholesterol than did autoxidation. These unique product ratios may be suitable indicators of past exposure to irradiation.