What question did this study set out to answer?

To determine how interleukin-5 and structural fibroblasts interact to regulate the survival, phenotypic density conversion, and cytotoxic function of human peripheral blood eosinophils.

October 1, 1989The Journal of ImmunologyOpen Access

IL-5-dependent conversion of normodense human eosinophils to the hypodense phenotype uses 3T3 fibroblasts for enhanced viability, accelerated hypodensity, and sustained antibody-dependent cytotoxicity.

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Authors

MRM.E. RothenbergCincinnati Children's Hospital Medical Center JPJ PetersenUtah State University RSR L StevensBrigham and Women's Hospital

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Implication

In vitro study demonstrates that fibroblasts accelerate IL-5-mediated hypodense conversion and boost cytotoxicity in human eosinophils, suggesting tissue factors regulate allergic pathology.

Key Points

To determine how interleukin-5 and structural fibroblasts interact to regulate the survival, phenotypic density conversion, and cytotoxic function of human peripheral blood eosinophils.
Cultured human peripheral blood eosinophils for 7 days with purified mouse IL-5 (1 pM) in the presence or absence of mouse 3T3 fibroblasts, evaluating both direct coculture and separation via 0.4-µm filters.
Assessed eosinophil survival, measured density distribution using density-gradient centrifugation, and quantified antibody-dependent cytotoxicity against helminthic larvae.
Eosinophil survival at 7 days was 0% without IL-5, 38 ± 12% with 1 pM IL-5 alone, 61 ± 14% with IL-5 and direct 3T3 fibroblast contact (p < 0.05, n=6), and 66 ± 7% when separated by a 0.4-µm filter (n=6).
All normodense eosinophils converted to a hypodense phenotype after 7 days of IL-5 exposure, and this conversion was accelerated in the presence of 3T3 fibroblasts.
Eosinophil-mediated larval cytotoxicity after 7 days was 10 ± 11% with IL-5 alone, 48 ± 17% with direct fibroblast coculture, and 31 ± 15% with filter-separated fibroblasts (n=5).

Cite This Study

Rothenberg et al. (1989) studied this question.

synapsesocial.com/papers/6a70604522b3175134db4fe1 https://doi.org/10.4049/jimmunol.143.7.2311

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Implication

In vitro study demonstrates that fibroblasts accelerate IL-5-mediated hypodense conversion and boost cytotoxicity in human eosinophils, suggesting tissue factors regulate allergic pathology.

IL-5-dependent conversion of normodense human eosinophils to the hypodense phenotype uses 3T3 fibroblasts for enhanced viability, accelerated hypodensity, and sustained antibody-dependent cytotoxicity.

Discussion

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Implication

Key Points

Cite This Study

IL-5-dependent conversion of normodense human eosinophils to the hypodense phenotype uses 3T3 fibroblasts for enhanced viability, accelerated hypodensity, and sustained antibody-dependent cytotoxicity.

Discussion

Member takes

Implication

Key Points

Cite This Study

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