What question did this study set out to answer?

To evaluate the analytical performance and proteomic capabilities of the Q Exactive HF-X benchtop Orbitrap mass spectrometer compared to its predecessor.

November 29, 2017Journal of Proteome ResearchOpen Access

Performance Evaluation of the Q Exactive HF-X for Shotgun Proteomics

View Full Paper

Ask AI

Bookmark

Authors

CKChristian D. KelstrupNovo Nordisk (Denmark)DBDorte B. Bekker‐JensenNovo Nordisk Foundation TATabiwang N. ArreyThermo Fisher Scientific (Germany)

Discussion

Member takes

Implication

Benchmarking study demonstrates enhanced peptide identification speed and sensitivity with the Q Exactive HF-X mass spectrometer, highlighting improved throughput for shotgun proteomics.

Key Points

To evaluate the analytical performance and proteomic capabilities of the Q Exactive HF-X benchtop Orbitrap mass spectrometer compared to its predecessor.
Benchmarked hardware and software modifications, including an upgraded peak-picking algorithm, a brighter ion source, and optimized ion transfer optics.
Assessed performance across short liquid chromatography-mass spectrometry (LC-MS) gradients, single-shot and multiplexed phosphoproteomic workflows, and data-independent acquisition (DIA) modes.
Achieved MS/MS acquisition speeds exceeding 40 Hz at 7,500 resolution, increasing peptide and protein identifications by up to 50% on short gradients (>1,000 unique peptides per minute).
Reached equivalent proteome coverage as the previous model using half the gradient run time or 10-fold lower sample input.
Quantified >5,000 phosphopeptides in single-shot 15-minute runs, 16,700 phosphopeptides across 10 conditions in 6 hours, and reproducibly identified 55,000 unique peptides spanning 5,900 proteins in a 30-minute DIA run.

Cite This Study

Kelstrup et al. (2017) studied this question.

synapsesocial.com/papers/6a706086e5469ee92be11d6e https://doi.org/10.1021/acs.jproteome.7b00602

View Full Paper

Ask AI

Bookmark

Implication

Benchmarking study demonstrates enhanced peptide identification speed and sensitivity with the Q Exactive HF-X mass spectrometer, highlighting improved throughput for shotgun proteomics.