Association of Porcine Swine Leukocyte Antigen (SLA) Haplotypes with B- and T-Cell Immune Response to Foot-and-Mouth Disease Virus (FMDV) Peptides

Figure 1. Frequencies of swine leukocyte antigen (SLA)-I and SLA-II allele-groups and Low-resolution (Lr) haplotypes in the cohort of 73 studied pigs. (A) Allele-groups at three SLA class I loci (SLA-1, SLA-2, and SLA-3) and three SLA class II loci (DRB-1, DQB-1, DQA). (B) SLA-I and SLA-II Lr-haplotypes (Table S1).

Figure 2. Correlations between SLA-I and SLA-II Low-resolution (Lr) haplotypes and the immune response elicited by dendrimer peptides in the 63 immunized animals (Group 0). Animal group composition as in Table S3. Antibody and cellular response values as in Table S2 and Figure S1. (A) Significant correlation of SLA-II Lr-haplotype and cellular response (χ2 α = 0.015 *, likelihood ratio chi-square (LR χ2) α = 0.038 *, Cv = 0.66). (B) No correlation of SLA-II Lr-haplotype and antibody response (χ2 α = 0.102, LR χ2 α = 0.634, Cv = 0.60). (C) Partially significant correlation of SLA-I Lr-haplotype and cellular response. (χ2 α = 0.053, LR χ2 α = 0.046 *, Cv = 0.59). (D) No correlation of SLA-I Lr-haplotype and antibody response (χ2 α = 0.065, LR χ2 α = 0.251, Cv = 0.57). VNT were categorized as 0: <0.0; 1: >0.9 and <1.5; 2: >1.5 and <1.8; 3: >1.8 and <2.4; 4: >2.4 and <3; 5: >3. IFN-γ secreting T cell values were categorized as: 0: <50; 1: >50 and <90; 2: >90 and <140; 3: >140.

Figure 3. Significant correlations between SLA-II Low-resolution (Lr) haplotypes and cellular response in immunized pigs from groups 1, 2, and 3. Animal group composition as in Table S3. Cellular response values as in Table S2 and Table 1. (A) Group 1. Significance: χ2 α = 0.022 *, LR χ2 α = 0.034 *, Fisher’s exact α = 0.003 *, Cv = 0.66. (B) Group 2. Significance: χ2 α = 0.010 *, LR χ2 α = 0.014 *, Fisher’s exact α = 0.001 *, Cv = 0.67. (C) Group 3. Significance: χ2 α = 0.004 *, LR χ2 α = 0.008 *, Fisher’s exact α = 0.001 *, Cv = 0.67. IFN-γ secreting T cell values were categorized as: 0: <50; 1: >50 and <90; 2: >90 and <140; 3: >140.

Figure 4. Significant correlation between SLA-I Low-resolution (Lr) haplotype and cellular response in pigs from group 1. Animal group composition as in Table S3, cellular response values as in Table S2 and Figure S1. Significance: χ2 α = 0.021 *, LR χ2 α = 0.039 *, Fisher’s exact α = 0.011 *, Cv = 0.64. IFN-γ secreting T cell values were categorized as: 0: <50; 1: >50 and <90; 2: >90 and <140; 3: >140.

Figure 5. Significant correlations between SLA-II and SLA-I+SLA-II Low-resolution (Lr) haplotypes and antibody response in group 3. Animal group composition as in Table S3, antibody response values as in Table S2 and Figure S1. (A) Antibody response and SLA-II haplotype. Significance: χ2 α = 0.010 *, LR χ2 α = 0.019 *, Fisher’s exact α = 0.011 *, Cv = 0.65. (B) Antibody response and SLA-I+SLA-II haplotype. Significance: χ2 α = 0.030 *, LR χ2 α = 0.022 *, Fisher’s exact α = 0.020 *, Cv = 0.72. VNT were categorized as: 0: <0.0; 1: >0.9 and <1.5; 2: >1.5 and <1.8; 3: >1.8 and <2.4; 4: >2.4 and <3; 5: >3.