Ligation of a Synthetic Peptide to the N Terminus of a Recombinant Protein Using Semisynthetic Protein trans ‐Splicing

Splice it! An active intein can be reconstituted from a recombinant C-terminal and a short synthetic N-terminal fragment. This allows ligation of a synthetic peptide to the N terminus of a recombinant protein of interest by protein trans-splicing and circumvents the requirement for a C-terminal thioester or other reactive groups common to chemical ligation approaches.