Netosis as a process involved in the consolidation of aneurysmal bone cysts in children using hemostatic agents

The study investigating the excessive formation of neutrophil extracellular traps (NETs) in neutrophils culture obtained while treatment of aneurysmal bone cysts using «Haemoblock», a hemostatic agent (Pul-Sar, Istra, Russian Federation), as a stimulator has been conducted. One hundred twenty-eight children with aneurysmal bone cysts (ABC) aged 6 to 15 years were included in the study. Sixty-three of the patients a clinical comparison group, underwent intralesional resection followed by alloplasty. Sixty-five of the patients made up a main clinical group who underwent minimally invasive surgery; 35 patients (A) and 30 children were treated by puncturing and administered a hemostatic agent (B). Ex vivo excessive NET generation was evaluated in neutrophil culture isolated from blood obtained during cyst puncturing and from peripheral venous blood in all children of the main clinical group. Neutrophils isolated from cysts had a statistically significantly increased ability to form extracellular traps compared to neutrophils from peripheral venous blood in response to stimulation by «Haemoblock», a hemostatic agent. Neutrophils in these patients formed mainly filamentous NETs. Thus, a modulating NETs formation plays a main role in providing intercellular interaction and proliferation of cells, including fibroblasts, leading to the closure of the cavity of aneurysmal bone cysts in children.