Neurobehavioral validation of a mild cognitive impairment model using Scopolamine in C57BL6/J mice

This is the authors' abstract. We don't add key points for this paper.

Persistent neuroinflammation is one of the major characteristic feature in almost every neurodegenerative diseases.The minor cellular perturbations in the brain cells progresses towards a cumulative inflammatory state which causes delay in the transmission of synaptic signals in the process of learning and memory formation.In the early stages of cognitive impairments, there are still some chances of reversing the inflammatory status by treating with neuromodulatory agents.In order to understand the early cellular changes in the brain tissue, we developed a mild cognitive impairment model using scopolamine as an inducer of neuroinflammation.We attempted to establish the duration of impairment after a 6 day consecutive administration of scopolamine; followed by changes in object based behavioural tests and estimation of biochemical changes in the brain tissue homogenates.The results show impairments in the spatial memory, location memory and pattern separation functions in the first three weeks after scopolamine administration.In the third week and the fourth week, the mice have been able to recover to their normal behavioural function.A similar trend is seen in the biochemical changes of the brain tissue homogenates indicates a time dependant decrease in the oxidative stress from the first week to fourth week post induction.Overall, the results indicate that the scopolamine induced neuroinflammation persists for 3-4 weeks after the administration and this provides us the window to explore the any treatment options to determine the short-term changes in the brain of mice with mild cognitive impairment.