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To investigate calcium-dependent mechanisms for chloride secretion in normal and cystic fibrosis airway epithelia.

Activation of an apical Cl- conductance by Ca2+ ionophores in cystic fibrosis airway epithelia

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To investigate calcium-dependent mechanisms for chloride secretion in normal and cystic fibrosis airway epithelia.
Nasal epithelia from normal and cystic fibrosis subjects were cultured on collagen membranes.
Responses to isoproterenol and calcium ionophores (A23187 and ionomycin) were measured using transepithelial and intracellular techniques.
Amiloride was used to pretreat cultures before testing ionophore effects.
Isoproterenol activated apical membrane chloride conductance in normal cultures but not in cystic fibrosis.
A23187 increased short-circuit current linked to chloride activation in both normal and cystic fibrosis epithelia.
Ionomycin also effectively stimulated chloride secretion in both groups, indicating preserved calcium-dependent pathways in cystic fibrosis.

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Randomized trial examines calcium-induced chloride secretion in cystic fibrosis airway cells, suggesting alternative pathways for treatment.

Willumsen et al. (1989) studied this question.

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Randomized trial examines calcium-induced chloride secretion in cystic fibrosis airway cells, suggesting alternative pathways for treatment.