What question did this study set out to answer?

To delineate the genetic and functional identity of intermediate CD14++CD16+ human monocytes in comparison to classical and nonclassical monocyte subsets.

July 30, 2011Blood

SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset

View Full Paper

Ask AI

Bookmark

To delineate the genetic and functional identity of intermediate CD14++CD16+ human monocytes in comparison to classical and nonclassical monocyte subsets.
Purified three monocyte subsets (classical CD14++CD16-, intermediate CD14++CD16+, and nonclassical CD14+CD16++) from human blood.
Profiled the transcriptomes of all three subsets using SuperSAGE combined with high-throughput tag sequencing, analyzing 5,487,603 total tags.
Performed Gene Ontology (GO) enrichment analysis to identify distinct biological processes and molecular markers specific to each subset.
Identified unique transcriptional identifiers that distinctively delineate intermediate CD14++CD16+ monocytes from the classical and nonclassical subsets.
Demonstrated functional gene enrichment in CD14++CD16+ monocytes for antigen presentation (CD74, HLA-DR, IFI30, CTSB), inflammation and monocyte activation (TGFB1, AIF1, PTPN6), and angiogenesis (TIE2, CD105).

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Transcriptomic profiling reveals a distinct molecular signature for intermediate CD14++CD16+ monocytes in human blood, highlighting specialized roles in antigen presentation and angiogenesis.

Cite This Study

Zawada et al. (2011) studied this question.

synapsesocial.com/papers/6a7070fb660549caf2c5058c https://doi.org/10.1182/blood-2011-01-326827

View Full Paper

Ask AI

Bookmark

Implication

Transcriptomic profiling reveals a distinct molecular signature for intermediate CD14++CD16+ monocytes in human blood, highlighting specialized roles in antigen presentation and angiogenesis.

SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset

Key Points

Discussion

Implication

Cite This Study

SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider