What question did this study set out to answer?

Design, synthesize, and evaluate the structural and thermodynamic consequences of incorporating one or two intrahelical disulfide cross-links into a model DNA duplex.

January 1, 1996View

Design, Synthesis, and Analysis of Disulfide Cross-Linked DNA Duplexes

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Authors

SOScott E. OsborneRutgers, The State University of New Jersey JVJens VölkerLukaskrankenhaus SSShawn Y. StevensRutgers, The State University of New Jersey

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Implication

Biophysical analysis demonstrates that intrahelical disulfide cross-links enhance DNA duplex stability and trap specific conformations, suggesting utility for studying nucleic acid dynamics.

Key Points

Design, synthesize, and evaluate the structural and thermodynamic consequences of incorporating one or two intrahelical disulfide cross-links into a model DNA duplex.
Synthesized DNA duplexes replacing 3'- and 5'-terminal bases with N3-thioethylthymidine, followed by air oxidation to induce disulfide bond formation.
Evaluated structural integrity using ultraviolet, circular dichroism, and NMR spectroscopies, alongside an Eco RI endonuclease cleavage assay.
Determined thermodynamic behavior and stability using optical thermal denaturation profiles and differential scanning calorimetry.
Disulfide cross-linking and thiol modifications did not alter overall duplex structure, preserving spectral characteristics and Eco RI cleavage capability.
Cross-links increased duplex thermal stability by shifting the molecularity of denaturation without producing an increase in enthalpy.
Intrahelical cross-linking resolved the conformational heterogeneity of the parent duplex by trapping it into a single, conformationally homogeneous state.

Cite This Study

Osborne et al. (1996) studied this question.

synapsesocial.com/papers/6a7073483ce530166bc2d4a4 https://doi.org/10.1021/ja962386c

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Implication

Biophysical analysis demonstrates that intrahelical disulfide cross-links enhance DNA duplex stability and trap specific conformations, suggesting utility for studying nucleic acid dynamics.

Design, Synthesis, and Analysis of Disulfide Cross-Linked DNA Duplexes

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Implication

Key Points

Cite This Study

Design, Synthesis, and Analysis of Disulfide Cross-Linked DNA Duplexes

Discussion

Member takes

Implication

Key Points

Cite This Study

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