Crystallization of elongation factor G from an extreme thermophile, Thermus thermophilus HB8

Elongation factor G is an important component of the protein-synthesizing system; its functional and structural aspects have been studied intensively. The G factor from Escherichiu coli is the most studied, but due to its structural instability no satisfactory crystals of this protein have been obtained yet. We have managed to grow single crystals of tryptic fragments of the G factor from E. cofi [ 11. Unfortunately, these crystals have a low radiation stability and a special approach should be developed for their study.