Determination of residual diaveridine and sulfaquinoxaline in hen's egg, chicken plasma and tissues by high-performance liquid chromatography.

A high-performance liquid chromatographic method for the determination of diaveridine (Dv) and sulfaquinoxaline (Sq) in hen's egg, chicken plasma, liver, kidney, heart, spleen, muscle, gizzard, skin and fat was developed using a C18 reversed-phase column and simple mobile phases. The pretreatments of Dv and Sq in such biological materials were classified into six groups. Detection limits were 0.02-0.04 ppm for Dv and 0.01-0.03 ppm for Sq. Average recoveries of Dv and Sq from egg and plasma were above 77%, and those from all other tissues ranged from 53 to 78%. The method was applied for residue analysis of Dv and Sq in egg yolk and albumen.