Effect of pro-urokinase, rt-PA and LMWH on the expression of urokinase type plasminogen activator system and tissue factor by HPAECs

Objective To study the effect of pro-urokinase,recombinant tissue type plasminogen activator (rt-PA) and low molecular weight heparin (LMWH) on the expression of urokinase type plasminogen activator system and tissue factor by HPAECs.Methods When the HPAECs reached confluent,starvate for 12h,and incubate the cells with different drugs for 12h.Cells were classified into 5 group according to different drug:control group,thrombin (1 IU/ml) group,thrombin (1 IU/ml) and pro-urokinase (150 IU/ml) group,thrombin (1 IU/ml) and rt-PA (150 IU/ml) group and thrombin (1 IU/ml) and LMWH (10 IU/ml) group.After incubation,collect the medium,and detect the content of u-PA,n-PAR,PAI-1 and TF.Results The content of PAI-1 in thrombin(1 IU/ml) group was statistically increased compared with control group,while the content of u-PA,u-PAR,and TF did not have great change.The content of u-PA and u-PAR in pro-urokinase group was statistically increased compared with control group,the content of PAI-1 was statistically decreased,while the content of TF did not have great change.The content of u-PA in rt-PA group was statistically increased compared with control group,the content of PAl-1 and TF was statistically decreased.The content of TF in LMWH group was statistically increased compared with control group,while the content of u-PA,u-PAR,and PAl-1 did not have great change.Conclusions Pro-urokinase,rt-PA and LMWH have direct effect on the fihrinolytic and anti-coagulant function of HPAECs.