Diluters for Bovine Semen. II. Effect of Milk Proteins upon Spermatozoan Livability

Since Thacker and Almquist However, studies with reconstituted nonfat dry milk solids, canned skimmilk, and evaporated whole milk have not yielded as encouraging results to date. Marion and Olson (16) and Bennett (2) found that nonfat dry milk solids prepared at Jow temperatures gave extremely poor results unless heated after reconstitution, "whereas reconstituted high temperature powders, without adclitional heating, gave results more nearly approaching those obtained 'with heated fluid milk and yolk-citrate. ,Tacquet and Cassou ( Collins (3) obtained fair livability but poor fertility results with semen diluted in evaporated milk reconstituted with an equal volume of distilled water. Thacker and Almquist (23) reported very poor spermatozoan livability in unheated fresh milks, as well as a reduction in motility survival when the milk was heated for an extended time in an uncovered vessel.