What question did this study set out to answer?

Determine whether the immune activation markers neopterin and 7,8-dihydroneopterin stimulate inducible nitric oxide synthase (iNOS) gene expression in vascular smooth muscle cells.

December 27, 1995FEBS Letters

Induction of inducible nitric oxide synthase expression by neopterin in vascular smooth muscle cells

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WSWolfgang SchobersbergerUMIT - Private Universität für Gesundheitswissenschaften, Medizinische Informatik und Technik GHGeorg HoffmannDeutsches Herzzentrum München JGJ. GroteUniversity of Bonn

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Implication

In vitro study reveals neopterin stimulates inducible nitric oxide synthase expression in rat vascular smooth muscle cells, suggesting a potential role in septic shock pathogenesis.

Key Points

Determine whether the immune activation markers neopterin and 7,8-dihydroneopterin stimulate inducible nitric oxide synthase (iNOS) gene expression in vascular smooth muscle cells.
Cultured rat vascular smooth muscle cells in vitro with neopterin or 7,8-dihydroneopterin.
Measured iNOS mRNA expression following exposure to 20 microM neopterin alone and in combination with 5 micrograms/ml Escherichia coli lipopolysaccharide.
Neopterin at 20 microM induced iNOS mRNA expression to 2.5 amol iNOS cDNA/micrograms total RNA, whereas 7,8-dihydroneopterin caused no induction.
Co-incubation of 20 microM neopterin with 5 micrograms/ml lipopolysaccharide demonstrated an additive effect, elevating iNOS cDNA concentration to 5.0 amol/micrograms total RNA.

Cite This Study

Schobersberger et al. (1995) studied this question.

synapsesocial.com/papers/6a707ec9439bab0cabc33124 https://doi.org/10.1016/0014-5793(95)01393-8

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Implication

In vitro study reveals neopterin stimulates inducible nitric oxide synthase expression in rat vascular smooth muscle cells, suggesting a potential role in septic shock pathogenesis.

Induction of inducible nitric oxide synthase expression by neopterin in vascular smooth muscle cells

Discussion

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Implication

Key Points

Cite This Study

Induction of inducible nitric oxide synthase expression by neopterin in vascular smooth muscle cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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