Why the study?
Hepatitis A outbreak surveillance requires rapid and accurate molecular diagnostic tools for HAV RNA detection, subgenotype identification, and sequence-based genotyping.
Does a new triplex rRT-PCR assay accurately detect and discriminate hepatitis A virus subgenotypes compared to standard methods in serum and plasma specimens?
Population
Serum and plasma specimens collected during unrelated HAV outbreaks in California and Michigan
Comparison
New triplex and singleplex rRT-PCR assays vs nested RT-PCR genotyping and ISO 15216-1 rRT-PCR
Design
Assay development and validation study
Authors
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May aid HAV outbreak surveillance; leaves open prospective validation before routine diagnostic adoption.
Does a new triplex rRT-PCR assay accurately detect and discriminate hepatitis A virus subgenotypes compared to standard methods in serum and plasma specimens?
The newly developed triplex and singleplex rRT-PCR assays provide highly accurate (>97%) and rapid detection and subgenotyping of hepatitis A virus, which can enhance public health outbreak surveillance.
Probert et al. (2019) studied this question.
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