What question did this study set out to answer?

To determine the recombinational role of the 28-bp psi sequence in plasmid pSC101 stability, plasmid multimer resolution, and chromosomal dif substitution.

June 1, 1994Journal of BacteriologyOpen Access

Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif

View Full Paper

Ask AI

Bookmark

Authors

FCFrançois CornetCentre National de la Recherche Scientifique IMI MortierLaboratoire de Microbiologie et Génétique Moléculaires JPJosette PatteLaboratoire de Microbiologie et Génétique Moléculaires

Discussion

Member takes

Implication

Molecular genetic analysis reveals that the pSC101 psi site resolves multimers and substitutes for chromosomal dif in Escherichia coli, indicating unprecedented recombination flexibility.

Key Points

To determine the recombinational role of the 28-bp psi sequence in plasmid pSC101 stability, plasmid multimer resolution, and chromosomal dif substitution.
Measured pSC101 inheritance and loss using a sensitive plasmid loss detection assay to evaluate psi-mediated stability.
Cloned the psi sequence into a p15A plasmid derivative and engineered an Escherichia coli strain by replacing the chromosomal dif site with psi.
Recombination at psi led to a moderate increase in plasmid pSC101 stability.
Multimer resolution on the plasmid derivative required accessory sequences, whereas chromosomal psi suppressed defects caused by dif deletion and mediated intermolecular exchanges.

Cite This Study

Cornet et al. (1994) studied this question.

synapsesocial.com/papers/6a7096d135aa2c282ce1e8e5 https://doi.org/10.1128/jb.176.11.3188-3195.1994

View Full Paper

Ask AI

Bookmark

Implication

Molecular genetic analysis reveals that the pSC101 psi site resolves multimers and substitutes for chromosomal dif in Escherichia coli, indicating unprecedented recombination flexibility.

Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif

Discussion

Member takes

Implication

Key Points

Cite This Study

Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider