Localization of atrial natriuretic peptide mRNA and immunoreactivity in the rat heart and human atrial appendage.
Population
Rat heart tissue sections and cultures (newborn rat), and human right atrial appendage sections
Comparison
In situ hybridization with 32P- and 35S-labeled… vs RNA probes identical to the coding sequence of…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Extends ANP expression sites to ventricular myocytes; leaves open its clinical relevance in human disease.
Structured PICO
P
Population
Rat heart tissue sections and cultures (newborn rat), and human right atrial appendage sections
I
Intervention
In situ hybridization with 32P- and 35S-labeled RNA probes for ANP mRNA
C
Comparator
RNA probes identical to the coding sequence of rat or human ANP mRNA (sense probes)
O
Outcome
Localization of preproatrial natriuretic peptide mRNA and ANP immunoreactivitysurrogate
This study provides unequivocal evidence for the expression of the ANP gene in both rat ventricles and atria, as well as human atrial appendages.
Cite This Study
Hamid et al. (1987) studied this question.