A pseudomonad has been isolated from soil which can utilize glutamate derived from the hydrolysis of folate or methotrexate (4-amino-N10-methylpteroylglutamic acid) as a source of carbon and nitrogen. During growth of the organism on folate, large quantities of pteroic acid accumulate in the growth medium. The enzyme responsible for the deamidation has been partially purified. It has a pH optimum of 7.3 and a Km toward methotrexate of 2.4 x 10-4 m. The reactivity of the enzyme toward a number of folate analogues has been studied.
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Levy et al. (1967) studied this question.
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