Summary. Continuous variations in circulating levels of plasma luteinizing hormone (LH) were measured over a 24-hr period in laying hens, by radioimmunoassay. A single LH peak, closely linked to the dark period, was associated with the occurrence of an ovulation. No other consistent changes in LH levels were found, apart from a small rise at the onset of darkness.
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Wilson et al. (1973) studied this question.
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