Why the study?
Developing a contamination-free, on-site, sensitive, specific, and low-cost nucleic acid detection platform is critical for infectious disease diagnosis and surveillance.
Does the RAA-Exo III colorimetric assay accurately and rapidly detect ASFV nucleic acids?
Does the RAA-Exo III colorimetric assay accurately and rapidly detect ASFV nucleic acids?
The developed RAA-Exo III colorimetric assay provides a rapid, sensitive, specific, and instrument-free platform for on-site detection of ASFV.
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May support low-cost on-site ASFV detection; leaves open prospective validation before surveillance use.
The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplification (RAA) with the exonuclease III ( Exo III)-assisted signal amplification into a platform for sensitive and specific detection of nucleic acids of African swine fever virus (ASFV). We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/μL in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. In addition, to avoid aerosol contamination, a one-tube RAA- Exo III colorimetric assay was also established for the accurate detection of ASFV in clinical samples. Taken together, we developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric-assay-based nucleic acid detection platform.
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Zhou et al. (2023) studied this question.
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