What question did this study set out to answer?

To clone and functionally characterize human casein kinase I epsilon (hCKI epsilon), a novel member of the CKI family involved in cellular regulation.

June 1, 1995Journal of Biological ChemistryOpen Access

Isolation and Characterization of Human Casein Kinase I∊ (CKI), a Novel Member of the CKI Gene Family

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To clone and functionally characterize human casein kinase I epsilon (hCKI epsilon), a novel member of the CKI family involved in cellular regulation.
Screened a human placental cDNA library to isolate and sequence the hCKI epsilon transcript.
Determined chromosomal localization, assessed gene expression via Northern blot across human cell lines, and tested kinase activity using CKI substrates and inhibitor CKI-7.
Performed genetic complementation assays by expressing hCKI epsilon in HRR25-deficient Saccharomyces cerevisiae strains.
Identified hCKI epsilon as a 416-amino acid polypeptide (47.3 kDa) mapping to chromosome 22q12-13, distinct from hCKI delta on chromosome 17q25.
Confirmed recombinant hCKI epsilon is an active kinase that phosphorylates CKI substrates and is specifically inhibited by CKI-7.
Demonstrated that expression of hCKI epsilon, but not hCKI alpha, rescues the slow-growth defect in yeast lacking the DNA-repair-associated kinase HRR25.

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Implication

Experimental study identifies human casein kinase I epsilon in placental tissue, indicating a conserved role in cellular growth and DNA repair pathways.

Cite This Study

Fish et al. (1995) studied this question.

synapsesocial.com/papers/6a70af8bac440176ef2959d9 https://doi.org/10.1074/jbc.270.25.14875

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Implication

Experimental study identifies human casein kinase I epsilon in placental tissue, indicating a conserved role in cellular growth and DNA repair pathways.

Isolation and Characterization of Human Casein Kinase I∊ (CKI), a Novel Member of the CKI Gene Family

Key Points

Discussion

Implication

Cite This Study

Isolation and Characterization of Human Casein Kinase I∊ (CKI), a Novel Member of the CKI Gene Family

Key Points

Discussion

Implication

Cite This Study

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