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June 24, 2021Scientific ReportsOpen Access

A link between pH homeostasis and colistin resistance in bacteria

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Authors

PPPradip R. PantaWDWilliam T. Doerrler

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Overview

Experimental study reveals that alkaline stress synergizes with colistin against diverse bacteria, suggesting cytoplasmic pH homeostasis is required for colistin resistance.

Key Points

  • To elucidate the molecular mechanism by which the membrane protein DbcA mediates extreme colistin resistance and assess the role of cytoplasmic pH homeostasis in antibiotic susceptibility.
  • Evaluated Burkholderia thailandensis wild-type and dbcA deletion mutants (∆dbcA) for sensitivity to alkaline pH and sodium bicarbonate exposure.
  • Tested colistin susceptibility across multiple Gram-negative and Gram-positive species under varying pH conditions, oxygen concentrations, and fermentable sugar (glucose) supplementation.
  • Deletion of dbcA conferred sensitivity to alkaline pH and sodium bicarbonate, demonstrating that DbcA promotes colistin resistance by sustaining cytoplasmic pH homeostasis in B. thailandensis.
  • Alkaline pH and sodium bicarbonate demonstrated synergistic bactericidal effects with colistin across extreme-resistant pathogens and the majority of Gram-negative and Gram-positive species tested.
  • Lowering media oxygen levels or supplementing fermentable glucose alleviated alkaline stress and elevated colistin resistance across tested bacteria by preventing cytoplasmic alkalinization.

Cite This Study

Panta et al. (2021) studied this question.

synapsesocial.com/papers/6a70aff8febe604dd7097604https://doi.org/10.1038/s41598-021-92718-7
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